引用本文：劉向輝;戈峰,.蚯蚓纖溶酶活性影響因子的研究[J].中國中藥雜志,2002,27(06):423.
中文摘要:目的 :對(duì)提取過程中影響蚯蚓纖溶酶活性的 5種因子進(jìn)行了分析 ,以提高纖溶酶的提取活性。方法 :分別采用 0.9%NaCl抽提、10%蔗糖抽提、75%酒精抽提出粗酶。酶活測(cè)定方法采用尿激酶瓊脂糖 纖維蛋白平板法。硒含量的測(cè)定方法采用 3,3′-二氨基聯(lián)苯胺比色法 ;砷含量的測(cè)定方法采用二乙氨基二硫代甲酸銀比色法。結(jié)果 :飼養(yǎng)在牛糞餌料中的蚯蚓纖溶酶活性比飼養(yǎng)于生活垃圾中的蚯蚓纖溶酶活性更高 ,蚯蚓體內(nèi)砷的含量與纖溶酶活性呈正相關(guān) ,而蚯蚓體內(nèi)硒的含量對(duì)纖溶酶活性影響不大。在采用 3種不同的蚯蚓纖溶酶的抽提方法中 ,75 %酒精抽提效率最高 ,0.9%氯化鈉次之 ,10%蔗糖最低 ;在蚯蚓纖溶酶純化過程中 ,透析比超濾對(duì)蚯蚓纖溶酶的活性影響更小一些。結(jié)論 :通過將蚯蚓飼養(yǎng)在適宜的餌料中 ,使用合適的提取手段和提純方法 ,可以提高蚯蚓纖溶酶的提取活性。
中文關(guān)鍵詞:蚯蚓纖溶酶活性 餌料 重金屬 抽提方法
點(diǎn)擊下載全文
Factors Influencing the Activity of Fibrinolytic Enzymes From Earthworm, Eisenia foetida
LIU Xiang hui;GE Feng
Abstract:Objective:To study the factors influencing the activity of fibrinolytic enzymes from earthworm and to obtain the better way to extract fibrinolytic enzymes as well as keep its optimum activity. Method:75% alcohol, 0.9%NaCl and 10% saccharose was used to extract the crude fibrinolytic enzymes from earthworm, the method of urokinase gelose fibrin plate was used to measure the activity of fibrinolytic enzymes from earthworm. and the method of 3,3′-diaminobezidine tetrahydrochloride colorimetry to was used measure the content of selenium. The method use ts of measuring the content of arsenic was silver diethyldithiocarbamate colorimetry. Result:The fibrinolytin of earthworms reared with cattle soils had higher activity than that reared with garbage. The arsenic in the earthworm′s body could improve the activity of earthworm′s fibrinolytin. However, the selenium had litter influence on it. Among the three methods of extraction, the 75% alcohol one was the most efficient , the 0.9% NaCl was next, and the 10% saccharose was the lowest. The influence of dialysis on the activity of fibrinolytin was less than that of ultrafiltration, when the earthworm′s fibrinolytin enzyme was further sublimated. Conclusion:The activity of the earthworm′s fibrinolytin will be increased earthworm is reared with the fitting baits and when appropriate methods, of extraction and purification are used.
keywords:activity of the earthworm′s fibrinolytin bait heavy metal method of extraction
查看全文
參考文獻(xiàn)(共7條):
［1］金鳴.蚯蚓溶栓作用進(jìn)展.中草藥,1994,24(1):45.
［2］路英華,金汝成,吳應(yīng)文,等. 鉅齒遠(yuǎn)蚓纖溶酶的分離純化及其若干性質(zhì).生物化學(xué)雜志,1998,4(2):166.
［3］徐建民,張國楨,陳松鶴,等. 赤子愛勝蚓纖維蛋白溶解酶的親和層析純化和生化特性.上海醫(yī)科大學(xué)學(xué)報(bào),1991,18(4):252.
［4］Nakajima N, Mihara H, Sumi H. Characterization of Potent Fibrinolytic Enzymes in Earthworm Lumbricus Rubellus. Biochem, 1993,57(10):1726.
［5］李群. 蚓激酶及其制劑活性的研究. 中國藥事, 1998,15(5):288.
［6］江蘇省連云港水產(chǎn)學(xué)校. 水產(chǎn)品檢驗(yàn)分析. 北京:中國農(nóng)業(yè)出版社,1995.
［7］劉向輝,戈峰,徐張紅,等. 亞硒酸鈉對(duì)蚯蚓的毒性及其蚓體富硒作用的研究.應(yīng)用與環(huán)境生物學(xué)報(bào),2001,7(5):457.
引證文獻(xiàn)(本文共被引2次):
[1] 武金霞,趙曉瑜.蚯蚓纖溶酶的研究進(jìn)展及趨勢(shì)[J].中國生化藥物雜志,2004,25(5).
[2] 解佰純,李景鵬.蚯蚓纖溶酶的研究進(jìn)展[J].東北農(nóng)業(yè)大學(xué)學(xué)報(bào),2006,37(6):834-837.
