醫(yī)學(xué)論文范文:外源磷脂酰乙醇胺誘導(dǎo)人宮頸癌HeLa細(xì)胞凋亡的研究

醫(yī)學(xué)論文范文:外源磷脂酰乙醇胺誘導(dǎo)人宮頸癌HeLa細(xì)胞凋亡的研究

【摘要】 目的 磷脂酰乙醇胺(phosphatidylethanolamine, PE)在細(xì)胞凋亡的早期由細(xì)胞膜內(nèi)側(cè)翻轉(zhuǎn)到外側(cè)，與磷脂酰絲氨酸共同成為細(xì)胞凋亡的早期信號(hào)，但是其在凋亡中的作用尚不清楚。本研究探討了PE對(duì)HeLa細(xì)胞凋亡的影響。方法 實(shí)驗(yàn)以人宮頸癌HeLa細(xì)胞系為材料，分別設(shè)置了空白對(duì)照組、0.125、0.25、0.5和1mmol/L PE處理組。以MTT檢測(cè)細(xì)胞生長(zhǎng)情況，PI�擦魇郊�(xì)胞儀法檢測(cè)細(xì)胞周期，Annexin V�睵I法檢測(cè)細(xì)胞凋亡。結(jié)果 與對(duì)照組相比，PE各處理組對(duì)HeLa細(xì)胞生長(zhǎng)的抑制作用呈劑量與時(shí)間依賴性，并誘導(dǎo)細(xì)胞凋亡發(fā)生，但不影響細(xì)胞周期。結(jié)論 PE通過誘導(dǎo)細(xì)胞凋亡而抑制HeLa細(xì)胞的生長(zhǎng)。

【關(guān)鍵詞】 磷脂酰乙醇胺;細(xì)胞凋亡;HeLa細(xì)胞

Apoptosis of human cervical cancer HeLa cells induced

by phosphatidylethanolamine

WANG Ai�瞴ing, HU Xiao�瞴an, LI Zong�瞗ang,LIU Li�瞴ing, NI Lei, YU Lin, SONG Tu�瞫heng

1. Key Laboratory of Environment and Genes Related to Diseases of Ministry ofEducation/ Genetics and Molecular Biology Department, Medical School ofXi�餫n Jiaotong University, Xi�餫n 710061; 2. the Second Affiliated Hospital,

Medical School of Xi�餫n Jiaotong Universitry, Xi�餫n 710004, China醫(yī).學(xué)全.在.線jfsoft.net.cn

ABSTRACT: Objective Phosphatidylethanolamine (PE) is an important phospholipid component in the cell membrane and is involved in the formation of membrane asymmetry. PE is exposed on the cell surface with phosphatidylserine during apoptosis. However, the effects of PE on cell apoptosis are not clear. In this study, we investigated effects of PE on apoptosis in human cervical cancer HeLa cells. Methods HeLa cells were used as the experiment material, and were divided into five groups: blank control group, and four treatment groups of 0.125, 0.25, 0.5 and 1 mmol/L PE, respectively. The cell growth was tested by MTT assay; the cell cycle and apoptosis were analyzed using flow cytometry. Results Compared with the control group, PE inhibited the growth of HeLa cells in all the treatment groups in dose�� and time�瞕ependent manners, and induced the apoptosis, but did not change the cell cycle. Conclusion PE inhibits the growth of HeLa cells by inducing the apoptosis.

KEY WORDS: phosphatidylethanolamine; apoptosis; HeLa cell

細(xì)胞膜的磷脂成分包括磷脂酰膽堿(phosphatidylcholine, PC)、磷脂酰乙醇胺(phosphatidylethanolamine, PE)、磷脂酰絲氨酸(phosphatidylserine, PS)和鞘磷脂(sphingomyelin, SM)[1]。在細(xì)胞膜上，磷脂呈不均等對(duì)稱分布，PC和SM分布于細(xì)胞膜的外側(cè)，而PE和PS分布于內(nèi)側(cè)[2��3]。氨基磷脂轉(zhuǎn)位酶(aminophospholipid translocase)參與了PE和PS的膜不對(duì)稱分布[4]。在正常情況下，氨基磷脂轉(zhuǎn)位酶可以將細(xì)胞膜外側(cè)的PE和PS轉(zhuǎn)為內(nèi)側(cè)。抑制氨基磷脂轉(zhuǎn)位酶活性可以誘導(dǎo)CNS衍化HN2��5和HOG細(xì)胞凋亡，并引起caspase��3激活[5]，提示細(xì)胞膜磷脂分布異�？梢砸�起細(xì)胞凋亡發(fā)生。細(xì)胞膜PS外翻已經(jīng)成為細(xì)胞凋亡的重要檢測(cè)指標(biāo)，并有研究證明PS外翻參與了細(xì)胞凋亡的誘導(dǎo)過程[6��7]。在細(xì)胞凋亡過程中，PE也發(fā)生外翻作用，并與PS一起參與了凋亡細(xì)胞脂筏的形成[8]。近年研究發(fā)現(xiàn)細(xì)胞內(nèi)PE結(jié)合蛋白(Raf kinase inhibitor protein, RKIP)表達(dá)增高，可以抑制Raf�睧RK1/2通路[9]。但是，關(guān)于PE是否能引起細(xì)胞增殖或凋亡尚不清晰，本研究組研究發(fā)現(xiàn)外源PE可以誘導(dǎo)宮頸癌HeLa細(xì)胞凋亡發(fā)生，現(xiàn)報(bào)道如下。

1 材料與方法

1.1 主要試劑及材料

RPMI 1640培養(yǎng)液和FBS胎牛血清購(gòu)自GIBCO公司;PE和PI購(gòu)自Sigma公司;Annexin V�睩ITC apoptosis detection kit購(gòu)自BD Bioscience公司。

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